Sulfated Glycans Inhibit the Interaction of MERS-CoV Receptor Binding Domain with Heparin

Authors:

 

J. Yang; Y. Song; W. Jin; K. Xia; G. C. Burnett; W. Qiao; J. T. Bates; V. H. Pomin; C. Wang; M. Qiao; R. J. Linhardt; J. S. Dordick; F. Zhang

Affiliation:

1The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China

2Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY 12180, USA

3College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China

4Department of Cell & Molecular Biology, The University of Mississippi Medical Center, Jackson, MS 39216, USA

5Department of BioMolecular Sciences, Research Institute of Pharmaceutical Sciences, The University of Mississippi, Oxford, MS 38677, USA

6Departments of Chemical and Biological Engineering, Rensselaer Polytechnic Institute, Troy, NY 12180, USA

 

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Description:

Middle East respiratory syndrome coronavirus (MERS-CoV) is a zoonotic virus with high contagion and mortality rates. Heparan sulfate proteoglycans (HSPGs) are ubiquitously expressed on the surface of mammalian cells. Owing to its high negatively charged property, heparan sulfate (HS) on the surface of host cells is used by many viruses as cofactor to facilitate viral attachment and initiate cellular entry. Therefore, inhibition of the interaction between viruses and HS could be a promising target to inhibit viral infection. In the current study, the interaction between the receptor-binding domain (RBD) of MERS-CoV and heparin was exploited to assess the inhibitory activity of various sulfated glycans such as glycosaminoglycans, marine-sourced glycans (sulfated fucans, fucosylated chondroitin sulfates, fucoidans, and rhamnan sulfate), pentosan polysulfate, and mucopolysaccharide using Surface Plasmon Resonance. We believe this study provides valuable insights for the development of sulfated glycan-based inhibitors as potential antiviral agents.

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Heparin

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Supplementary Materials Supplementary Materials The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/v16020237/s1, Figure S1: IC50 measurement of the inhibition of MERS RBD binding to heparin using solution competition SPR by seaweed-sourced sulfated glycans. zip 301.73 KB Login to download