Glyco Property List
Pooled literature properties (glycanDB). Optional filters: ?dataset=uv_vis&property_key=...
GET /api/gdd/glyco-properties/?format=api&page=34
{ "count": 839, "next": "http://glycodata.org/api/gdd/glyco-properties/?format=api&page=35", "previous": "http://glycodata.org/api/gdd/glyco-properties/?format=api&page=33", "results": [ { "id": 661, "dataset": "heparin", "entity": "heparin decasaccharide (dp10, canonical)", "entity_group": "", "entity_class": "heparin oligosaccharide", "property": "protein binding affinity (Kd)", "property_key": "binding_affinity", "value": "1.3", "unit": "µM", "value_reported": "1.3", "unit_reported": "µM", "method": "Experiment", "method_detail": "Kd vs VEGF165 by SPR solution affinity assay (oligosaccharide 10b)", "conditions": "Kd vs VEGF165 by SPR solution affinity assay (oligosaccharide 10b)", "conditions_detail": "Kd vs VEGF165 by SPR solution affinity assay (oligosaccharide 10b) | partner: VEGF165 | technique: SPR", "doi": "10.1042/BSR20110077", "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC3190640/", "year": "2012", "confidence": "high", "comments": "\"10b (decasaccharide) ... VEGF165 1.3 ± 0.3 µM (SPR solution affinity)\" — Decasaccharide vs VEGF165; Table 2 [Zhao W, McCallum SA, Xiao Z, Zhang F, Linhardt RJ, 2012, confidence: high]" }, { "id": 662, "dataset": "heparin", "entity": "heparin tetradecasaccharide (dp14)", "entity_group": "", "entity_class": "heparin oligosaccharide", "property": "protein binding affinity (Kd)", "property_key": "binding_affinity", "value": "0.09", "unit": "µM", "value_reported": "90", "unit_reported": "nM", "method": "Experiment", "method_detail": "Kd vs VEGF165 by SPR solution affinity assay (oligosaccharide 14b)", "conditions": "Kd vs VEGF165 by SPR solution affinity assay (oligosaccharide 14b)", "conditions_detail": "Kd vs VEGF165 by SPR solution affinity assay (oligosaccharide 14b) | partner: VEGF165 | technique: SPR", "doi": "10.1042/BSR20110077", "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC3190640/", "year": "2012", "confidence": "high", "comments": "\"14b (tetradecasaccharide) ... VEGF165 90 ± 5 nM; a tetradecasaccharide displayed an effective binding affinity to VEGF165 comparable to unfractionated heparin\" — dp14 binds VEGF165 comparably to UFH; Table 2 [Zhao W, McCallum SA, Xiao Z, Zhang F, Linhardt RJ, 2012, confidence: high]" }, { "id": 663, "dataset": "heparin", "entity": "heparin hexadecasaccharide (dp16)", "entity_group": "", "entity_class": "heparin oligosaccharide", "property": "protein binding affinity (Kd)", "property_key": "binding_affinity", "value": "0.113", "unit": "µM", "value_reported": "113", "unit_reported": "nM", "method": "Experiment", "method_detail": "Kd vs VEGF165 by SPR solution affinity assay (oligosaccharide 16b)", "conditions": "Kd vs VEGF165 by SPR solution affinity assay (oligosaccharide 16b)", "conditions_detail": "Kd vs VEGF165 by SPR solution affinity assay (oligosaccharide 16b) | partner: VEGF165 | technique: SPR", "doi": "10.1042/BSR20110077", "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC3190640/", "year": "2012", "confidence": "high", "comments": "\"16b (hexadecasaccharide) ... VEGF165 113 ± 26 nM (SPR solution affinity)\" — dp16 vs VEGF165; Table 2 [Zhao W, McCallum SA, Xiao Z, Zhang F, Linhardt RJ, 2012, confidence: high]" }, { "id": 664, "dataset": "heparin", "entity": "heparan sulfate (sulfated domains)", "entity_group": "", "entity_class": "heparan sulfate", "property": "protein binding affinity (Kd)", "property_key": "binding_affinity", "value": "0.03", "unit": "µM", "value_reported": "30", "unit_reported": "nM", "method": "Experiment", "method_detail": "Kd of SDF-1/CXCL12 binding to the sulfated regions of heparan sulfate", "conditions": "Kd of SDF-1/CXCL12 binding to the sulfated regions of heparan sulfate", "conditions_detail": "Kd of SDF-1/CXCL12 binding to the sulfated regions of heparan sulfate | partner: SDF-1 / CXCL12 (stromal cell-derived factor-1) | technique: binding assay", "doi": "10.1074/jbc.M311439200", "source_url": "https://www.sciencedirect.com/science/article/pii/S0021925820680589", "year": "2004", "confidence": "medium", "comments": "\"SDF-1 binds with high affinity (Kd of 30 nM) to the sulfated regions of heparan sulfate (HS)\" — SDF-1/CXCL12-HS Kd; value from web search summary of this SDF-1/HS paper [Sadir R, Imberty A, Baleux F, Lortat-Jacob H, 2004, confidence: medium]" }, { "id": 665, "dataset": "heparin", "entity": "heparin octasaccharide (dp8)", "entity_group": "", "entity_class": "heparin oligosaccharide", "property": "protein binding affinity (Kd)", "property_key": "binding_affinity", "value": "34", "unit": "µM", "value_reported": "34", "unit_reported": "µM", "method": "Experiment", "method_detail": "Apparent Kd for 1:1 complex of heparin octasaccharide with disulfide-trapped CXCL1 dimer, by solution NMR chemical-shift-perturbation titration", "conditions": "Apparent Kd for 1:1 complex of heparin octasaccharide with disulfide-trapped CXCL1 dimer, by solution NMR chemical-shift-perturbation titration", "conditions_detail": "Apparent Kd for 1:1 complex of heparin octasaccharide with disulfide-trapped CXCL1 dimer, by solution NMR chemical-shift-perturbation titration | partner: CXCL1 (chemokine) dimer | technique: NMR", "doi": "10.1074/jbc.M113.492579", "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC3757178/", "year": "2013", "confidence": "high", "comments": "\"The calculated apparent dissociation constant for a 1:1 complex is 34 ± 6 µm\" — Heparin octasaccharide vs CXCL1 dimer (dCXCL1); NMR titration [Poluri KM, Joseph PRB, Sawant KV, Rajarathnam K, 2013, confidence: high]" }, { "id": 666, "dataset": "heparin", "entity": "heparin", "entity_group": "", "entity_class": "heparin (UFH)", "property": "protein binding affinity (Kd)", "property_key": "binding_affinity", "value": "0.055", "unit": "µM", "value_reported": "55", "unit_reported": "nM", "method": "Experiment", "method_detail": "KD of full-length monomeric SARS-CoV-2 spike protein binding to immobilized heparin by SPR", "conditions": "KD of full-length monomeric SARS-CoV-2 spike protein binding to immobilized heparin by SPR", "conditions_detail": "KD of full-length monomeric SARS-CoV-2 spike protein binding to immobilized heparin by SPR | partner: SARS-CoV-2 spike protein (full-length monomeric) | technique: SPR", "doi": "10.1021/acscentsci.1c00010", "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8227597/", "year": "2021", "confidence": "high", "comments": "\"The full-length monomeric spike protein showed a much higher binding affinity with a KD value of 55 nM\" — SARS-CoV-2 spike (monomeric) vs heparin; SPR [Liu L, Chopra P, Li X, Bouwman KM, Tompkins SM, et al., 2021, confidence: high]" }, { "id": 667, "dataset": "heparin", "entity": "heparin", "entity_group": "", "entity_class": "heparin (UFH)", "property": "protein binding affinity (Kd)", "property_key": "binding_affinity", "value": "0.064", "unit": "µM", "value_reported": "64", "unit_reported": "nM", "method": "Experiment", "method_detail": "KD of trimeric SARS-CoV-2 spike protein binding to immobilized heparin by SPR", "conditions": "KD of trimeric SARS-CoV-2 spike protein binding to immobilized heparin by SPR", "conditions_detail": "KD of trimeric SARS-CoV-2 spike protein binding to immobilized heparin by SPR | partner: SARS-CoV-2 spike protein (trimeric) | technique: SPR", "doi": "10.1021/acscentsci.1c00010", "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8227597/", "year": "2021", "confidence": "high", "comments": "\"The trimeric spike protein displayed a similar binding affinity (KD = 64 nM)\" — SARS-CoV-2 spike (trimeric) vs heparin; SPR [Liu L, Chopra P, Li X, Bouwman KM, Tompkins SM, et al., 2021, confidence: high]" }, { "id": 668, "dataset": "heparin", "entity": "heparin", "entity_group": "", "entity_class": "heparin (UFH)", "property": "protein binding affinity (Kd)", "property_key": "binding_affinity", "value": "1", "unit": "µM", "value_reported": "1", "unit_reported": "µM", "method": "Experiment", "method_detail": "KD of SARS-CoV-2 receptor binding domain (RBD) binding to immobilized heparin by SPR", "conditions": "KD of SARS-CoV-2 receptor binding domain (RBD) binding to immobilized heparin by SPR", "conditions_detail": "KD of SARS-CoV-2 receptor binding domain (RBD) binding to immobilized heparin by SPR | partner: SARS-CoV-2 spike receptor binding domain (RBD) | technique: SPR", "doi": "10.1021/acscentsci.1c00010", "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC8227597/", "year": "2021", "confidence": "high", "comments": "\"The RBD domain binds to heparin with a moderate affinity having a KD value of ~1 µM\" — SARS-CoV-2 spike RBD vs heparin; SPR [Liu L, Chopra P, Li X, Bouwman KM, Tompkins SM, et al., 2021, confidence: high]" }, { "id": 669, "dataset": "heparin", "entity": "heparin (end-chain immobilized)", "entity_group": "", "entity_class": "heparin (UFH)", "property": "protein binding affinity (Kd)", "property_key": "binding_affinity", "value": "0.021", "unit": "µM", "value_reported": "21", "unit_reported": "nM", "method": "Experiment", "method_detail": "Kd of annexin V binding to end-chain immobilized heparin by SPR", "conditions": "Kd of annexin V binding to end-chain immobilized heparin by SPR", "conditions_detail": "Kd of annexin V binding to end-chain immobilized heparin by SPR | partner: annexin V | technique: SPR", "doi": "10.1016/S0014-5793(99)00245-8", "source_url": "https://www.sciencedirect.com/science/article/pii/S0014579399002458", "year": "1999", "confidence": "medium", "comments": "\"Surface plasmon resonance analysis afforded a Kd of approximately 21 nM for the interaction of annexin V with end-chain immobilized heparin\" — Annexin V vs heparin; value via web search summary (full text 403) [Capila I, VanderNoot VA, Mealy TR, Seaton BA, Linhardt RJ, 1999, confidence: medium]" }, { "id": 670, "dataset": "heparin", "entity": "heparan sulfate (end-chain immobilized)", "entity_group": "", "entity_class": "heparan sulfate", "property": "protein binding affinity (Kd)", "property_key": "binding_affinity", "value": "0.049", "unit": "µM", "value_reported": "49", "unit_reported": "nM", "method": "Experiment", "method_detail": "Kd of annexin V binding to end-chain immobilized heparan sulfate by SPR", "conditions": "Kd of annexin V binding to end-chain immobilized heparan sulfate by SPR", "conditions_detail": "Kd of annexin V binding to end-chain immobilized heparan sulfate by SPR | partner: annexin V | technique: SPR", "doi": "10.1016/S0014-5793(99)00245-8", "source_url": "https://www.sciencedirect.com/science/article/pii/S0014579399002458", "year": "1999", "confidence": "medium", "comments": "\"a Kd of approximately 49 nM for the interaction with end-chain immobilized heparan sulfate\" — Annexin V vs heparan sulfate; value via web search summary (full text 403) [Capila I, VanderNoot VA, Mealy TR, Seaton BA, Linhardt RJ, 1999, confidence: medium]" }, { "id": 671, "dataset": "heparin", "entity": "heparin", "entity_group": "", "entity_class": "heparin (UFH)", "property": "protein binding affinity (Kd)", "property_key": "binding_affinity", "value": "0.032", "unit": "µM", "value_reported": "32", "unit_reported": "nM", "method": "Experiment", "method_detail": "Apparent Kd of annexin II tetramer binding to heparin, stoichiometry 11 mol tetramer per mol heparin", "conditions": "Apparent Kd of annexin II tetramer binding to heparin, stoichiometry 11 mol tetramer per mol heparin", "conditions_detail": "Apparent Kd of annexin II tetramer binding to heparin, stoichiometry 11 mol tetramer per mol heparin | partner: annexin II tetramer | technique: binding assay", "doi": "10.1074/jbc.272.24.15093", "source_url": "https://www.sciencedirect.com/science/article/pii/S002192581839450X", "year": "1997", "confidence": "medium", "comments": "\"Annexin II tetramer bound to heparin with an apparent Kd of 32 ± 6 nM with a stoichiometry of 11 ± 0.9 mol of annexin II tetramer per mol of heparin\" — Annexin II tetramer vs heparin; value via web search summary (full text 403) [Kassam G, Manro A, Braat CE, Louie P, Fitzpatrick SL, Waisman DM, 1997, confidence: medium]" }, { "id": 672, "dataset": "heparin", "entity": "heparin", "entity_group": "", "entity_class": "heparin (UFH)", "property": "protein binding affinity (Kd)", "property_key": "binding_affinity", "value": "0.0031", "unit": "µM", "value_reported": "3.1", "unit_reported": "nM", "method": "Experiment", "method_detail": "Kd of heparin binding to RAGE (receptor for advanced glycation endproducts)", "conditions": "Kd of heparin binding to RAGE (receptor for advanced glycation endproducts)", "conditions_detail": "Kd of heparin binding to RAGE (receptor for advanced glycation endproducts) | partner: RAGE (receptor for advanced glycation endproducts) | technique: binding assay", "doi": "10.1021/acs.jmedchem.7b00058", "source_url": "https://pubs.acs.org/doi/10.1021/acs.jmedchem.7b00058", "year": "2022", "confidence": "medium", "comments": "\"chondroitin sulfate E, heparan sulfate, and heparin have high affinity for RAGE with Kd values of 0.2, 0.6, and 3.1 nM, respectively\" — Heparin vs RAGE; value via web search summary [Singh H, Agrawal DK, 2022, confidence: medium]" }, { "id": 673, "dataset": "heparin", "entity": "heparan sulfate", "entity_group": "", "entity_class": "heparan sulfate", "property": "protein binding affinity (Kd)", "property_key": "binding_affinity", "value": "0.0006", "unit": "µM", "value_reported": "0.6", "unit_reported": "nM", "method": "Experiment", "method_detail": "Kd of heparan sulfate binding to RAGE", "conditions": "Kd of heparan sulfate binding to RAGE", "conditions_detail": "Kd of heparan sulfate binding to RAGE | partner: RAGE (receptor for advanced glycation endproducts) | technique: binding assay", "doi": "10.1021/acs.jmedchem.7b00058", "source_url": "https://pubs.acs.org/doi/10.1021/acs.jmedchem.7b00058", "year": "2022", "confidence": "medium", "comments": "\"chondroitin sulfate E, heparan sulfate, and heparin have high affinity for RAGE with Kd values of 0.2, 0.6, and 3.1 nM, respectively\" — Heparan sulfate vs RAGE; value via web search summary [Singh H, Agrawal DK, 2022, confidence: medium]" }, { "id": 674, "dataset": "heparin", "entity": "heparin (low-affinity, full-length)", "entity_group": "", "entity_class": "heparin (UFH)", "property": "protein binding affinity (Kd)", "property_key": "binding_affinity", "value": "6", "unit": "µM", "value_reported": "6", "unit_reported": "µM", "method": "Experiment", "method_detail": "Intrinsic dissociation constant (KD,obs ~ 6-10 microM) of thrombin-heparin interaction at physiological ionic strength", "conditions": "Intrinsic dissociation constant (KD,obs ~ 6-10 microM) of thrombin-heparin interaction at physiological ionic strength", "conditions_detail": "Intrinsic dissociation constant (KD,obs ~ 6-10 microM) of thrombin-heparin interaction at physiological ionic strength | partner: thrombin | technique: fluorescence/SPR (bulk)", "doi": "10.1016/j.abb.2008.03.044", "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC3461310/", "year": "2008", "confidence": "medium", "comments": "\"The thrombin-heparin interaction was characterized by an intrinsic dissociation constant (KD,obs) of 6-10 microM at physiological ionic strength\" — Thrombin-heparin (exosite II) low-affinity binding; value via web search summary; range 6-10 uM (lower bound recorded) [Verhamme IM, Bock PE, 2008, confidence: medium]" }, { "id": 675, "dataset": "heparin", "entity": "heparin", "entity_group": "", "entity_class": "heparin (UFH)", "property": "protein binding affinity (Kd)", "property_key": "binding_affinity", "value": "20", "unit": "µM", "value_reported": "20", "unit_reported": "µM", "method": "Experiment", "method_detail": "Equilibrium dissociation constant K_HCII(Hep) for heparin binding to heparin cofactor II", "conditions": "Equilibrium dissociation constant K_HCII(Hep) for heparin binding to heparin cofactor II", "conditions_detail": "Equilibrium dissociation constant K_HCII(Hep) for heparin binding to heparin cofactor II | partner: heparin cofactor II (HCII) | technique: fluorescence", "doi": "10.1016/j.abb.2008.03.044", "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC3461310/", "year": "2008", "confidence": "medium", "comments": "\"KHCII(Hep) = 20 ± 5 µM, similar to the values reported independently by us and others\" — Heparin cofactor II vs heparin; value via web search summary [Verhamme IM, Bock PE, 2008, confidence: medium]" }, { "id": 676, "dataset": "heparin", "entity": "heparin (porcine intestinal)", "entity_group": "", "entity_class": "heparin (UFH)", "property": "disaccharide composition", "property_key": "disaccharide_composition", "value": "72.6", "unit": "%", "value_reported": "72.6", "unit_reported": "%", "method": "Experiment", "method_detail": "mol% of TriS disaccharide (UA2S-GlcNS6S) in unfractionated heparin (UFH), LC-MS disaccharide analysis after heparin lyase digestion", "conditions": "mol% of TriS disaccharide (UA2S-GlcNS6S) in unfractionated heparin (UFH), LC-MS disaccharide analysis after heparin lyase digestion", "conditions_detail": "mol% of TriS disaccharide (UA2S-GlcNS6S) in unfractionated heparin (UFH), LC-MS disaccharide analysis after heparin lyase digestion | unit id: TriS (UA2S-GlcNS6S) | source: porcine intestinal", "doi": "10.1002/jps.23939", "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC3998821/", "year": "2014", "confidence": "high", "comments": "\"TriS...72.6 ± 0.3\" — UFH starting material; dominant trisulfated unit [Li Fu, Fuming Zhang, Guoyun Li, Akihiro Onishi, Ujjwal Bhaskar, Peilong Sun, Robert J. Linhardt, 2014, confidence: high]" }, { "id": 677, "dataset": "heparin", "entity": "heparin (porcine intestinal)", "entity_group": "", "entity_class": "heparin (UFH)", "property": "disaccharide composition", "property_key": "disaccharide_composition", "value": "14.1", "unit": "%", "value_reported": "14.1", "unit_reported": "%", "method": "Experiment", "method_detail": "mol% of NS6S disaccharide (UA-GlcNS6S) in UFH, LC-MS disaccharide analysis", "conditions": "mol% of NS6S disaccharide (UA-GlcNS6S) in UFH, LC-MS disaccharide analysis", "conditions_detail": "mol% of NS6S disaccharide (UA-GlcNS6S) in UFH, LC-MS disaccharide analysis | unit id: NS6S (UA-GlcNS6S) | source: porcine intestinal", "doi": "10.1002/jps.23939", "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC3998821/", "year": "2014", "confidence": "high", "comments": "\"ΔDi-NS6S...14.1 ± 0.8\" [Li Fu, Fuming Zhang, Guoyun Li, Akihiro Onishi, Ujjwal Bhaskar, Peilong Sun, Robert J. Linhardt, 2014, confidence: high]" }, { "id": 678, "dataset": "heparin", "entity": "heparin (porcine intestinal)", "entity_group": "", "entity_class": "heparin (UFH)", "property": "disaccharide composition", "property_key": "disaccharide_composition", "value": "5.4", "unit": "%", "value_reported": "5.4", "unit_reported": "%", "method": "Experiment", "method_detail": "mol% of NS2S disaccharide (UA2S-GlcNS) in UFH, LC-MS disaccharide analysis", "conditions": "mol% of NS2S disaccharide (UA2S-GlcNS) in UFH, LC-MS disaccharide analysis", "conditions_detail": "mol% of NS2S disaccharide (UA2S-GlcNS) in UFH, LC-MS disaccharide analysis | unit id: NS2S (UA2S-GlcNS) | source: porcine intestinal", "doi": "10.1002/jps.23939", "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC3998821/", "year": "2014", "confidence": "high", "comments": "\"ΔDi-NS2S...5.4 ± 0.2\" [Li Fu, Fuming Zhang, Guoyun Li, Akihiro Onishi, Ujjwal Bhaskar, Peilong Sun, Robert J. Linhardt, 2014, confidence: high]" }, { "id": 679, "dataset": "heparin", "entity": "heparin (porcine intestinal)", "entity_group": "", "entity_class": "heparin (UFH)", "property": "disaccharide composition", "property_key": "disaccharide_composition", "value": "1.9", "unit": "%", "value_reported": "1.9", "unit_reported": "%", "method": "Experiment", "method_detail": "mol% of NS disaccharide (UA-GlcNS) in UFH, LC-MS disaccharide analysis", "conditions": "mol% of NS disaccharide (UA-GlcNS) in UFH, LC-MS disaccharide analysis", "conditions_detail": "mol% of NS disaccharide (UA-GlcNS) in UFH, LC-MS disaccharide analysis | unit id: NS (UA-GlcNS) | source: porcine intestinal", "doi": "10.1002/jps.23939", "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC3998821/", "year": "2014", "confidence": "high", "comments": "\"ΔDi-NS...1.9 ± 0.1\" [Li Fu, Fuming Zhang, Guoyun Li, Akihiro Onishi, Ujjwal Bhaskar, Peilong Sun, Robert J. Linhardt, 2014, confidence: high]" }, { "id": 680, "dataset": "heparin", "entity": "heparin (porcine intestinal)", "entity_group": "", "entity_class": "heparin (UFH)", "property": "disaccharide composition", "property_key": "disaccharide_composition", "value": "0.8", "unit": "%", "value_reported": "0.8", "unit_reported": "%", "method": "Experiment", "method_detail": "mol% of unsulfated 0S disaccharide (UA-GlcNAc) in UFH, LC-MS disaccharide analysis", "conditions": "mol% of unsulfated 0S disaccharide (UA-GlcNAc) in UFH, LC-MS disaccharide analysis", "conditions_detail": "mol% of unsulfated 0S disaccharide (UA-GlcNAc) in UFH, LC-MS disaccharide analysis | unit id: 0S / unsulfated (UA-GlcNAc) | source: porcine intestinal", "doi": "10.1002/jps.23939", "source_url": "https://pmc.ncbi.nlm.nih.gov/articles/PMC3998821/", "year": "2014", "confidence": "high", "comments": "\"ΔDi-0S...0.8 ± 0.2\" — very low unsulfated content is characteristic of heparin vs HS [Li Fu, Fuming Zhang, Guoyun Li, Akihiro Onishi, Ujjwal Bhaskar, Peilong Sun, Robert J. Linhardt, 2014, confidence: high]" } ] }